One Two
Prof. Dr. med. Alexander Pfeifer

of the Institute of Pharmacology and Toxicology
Rheinische Friedrich-Wilhelms-University Bonn
BMZ 2G014/15
Venusberg-Campus 1
D-53127 Bonn

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Tel: +49-228-287-51300
Fax: +49-228-287-51301

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Pfeifer group

Prof. Dr. med. Alexander Pfeifer


Key research interests

  • NO/cGMP-signaling cascade – cGMP-dependent proteinkinase; cell- and animal models
  • Metabolism: differentiation and function of brown and white adipose tissue
  • Viral vectors: focus lentiviral vectors; lentiviral transgenesis (generation of transgenic animals using lentiviral gene transfer)
  • RNA-interference: siRNA-based therapies
  • Drug targeting - nanomedicine


  • Viral vector platform
  • Model systems: lentiviral transgenesis; knockout-mice
  • State-of-the-art molecular biology

Important publications


  • Sanyal A, Naumann J, Hoffmann LS, Chabowska-Kita A, Ehrlund A, Schlitzer A, Arner P, Blüher M, Pfeifer A (2017) Interplay between Obesity-Induced Inflammation and cGMP Signaling in White Adipose Tissue. Cell Rep. 2017 Jan 3;18(1):225-236. doi: 10.1016/j
  • Chen Y, Buyel JJ, Hanssen MJ, Siegel F, Pan R, Naumann J, Schell M, van der Lans A, Schlein C, Froehlich H, Heeren J, Virtanen KA, van Marken Lichtenbelt W, Pfeifer A. (2016) Exosomal microRNA miR-92a concentration in serum reflects human brown fat activity. Nat Commun. 2016 Apr 27;7:11420. doi: 10.1038/ncomms11420.
  • Klepac K, Kilić A, Gnad T, Brown LM, Herrmann B, Wilderman A, Balkow A, Glöde A, Simon K, Lidell ME, Betz MJ, Enerbäck S, Wess J, Freichel M, Blüher M, König G, Kostenis E, Insel PA, Pfeifer A. (2016) The Gq signalling pathway inhibits brown and beige adipose tissue. Nat Commun. 2016 Mar 9;7:10895. doi: 10.1038/ncomms10895
  • Hoffmann LS, Etzrodt J, Willkomm L, Sanyal A, Scheja L, Fischer AW, Stasch JP, Bloch W, Friebe A, Heeren J, Pfeifer A. (2015) Stimulation of soluble guanylyl cyclase protects against obesity by recruiting brown adipose tissue, Nature Communications, DOI: 10.1038/ncomms8235
  • Gnad T, Scheibler S, von Kügelgen I, Scheele C, Kilic A, Glöde A, Hoffmann LS, Reverte L, Horn P, Mutlu S, El-Tayeb A, Kranz M, Deuther-Conrad W, Brust P,  Lidell ME, Betz M, Enerbäck S, Schrader J, Yegutkin GG, Müller CE, Pfeifer A (2014). Adenosine activates brown adipose tissue and recruits beige adipocytes via A2A receptors. Nature, doi: 10.1038/nature13816

  • Chen Y, Siegel F, Kipschull S, Haas B, Fröhlich H, Meister G, Pfeifer A. miR-155 regulates differentiation of brown and beige adipocytes via a bistable circuit.
    Nat Commun. 2013 Apr 23;4:1769. doi: 10.1038/ncomms2742.[fulltext]
  • Mitschke MM., Hoffmann LS., Gnad T., Scholz D., Kruithoff K., Mayer P., Haas B., Sassmann A., Pfeifer A., Kilic A. (2013). Increased cGMP promotes healthy expansion and browning of white adipose tissue. The FASEB Journal, January 9 [Epub ahead of print]
  • Jennissen K., Siegel F., Liebig-Gonglach M., Hermann MR., Kipschull S., van Dooren S., Kunz WS., Fässler R., Pfeifer A. (2012). A VASP-Rac-Soluble Guanyly Cyclase Pathway Controls cGMP Production in Adipocytes. Science Signaling 5 (239), ra62. [free full text] [abstract] [summary]
  • Haas, B., Jennissen K., Mayer, P., Scholz, D., Berriel Diaz, M., Bloch, W., Herzig, S., Fässler, R., Pfeifer A. (2009). Protein kinase G controls brown fat cell differentiation and mitochondrial biogenesis. Science Signaling 2009; Dec 1;2(99):ra78. [free full text]   [abstract]  [summary] [podcast]
  • Hofmann, A., Wenzel, D., Becher, UM., Freitag, DF., Klein, AM., Eberbeck, D., Schulte, M., Zimmermann, K., Bergemann, C., Gleich, B., Roell, W., Weyh, T., Trahms, L., Nickenig, G., Fleischmann, BK., Pfeifer, A. (2009). Combined targeting of lentiviral vectors and positioning of transduced cells by magnetic nanoparticles. Proc. Natl. Acad. Sci. U S A. 2009; 106(1): 44-9
  • Massoumi, R., Chmielarska, K., Hennecke, K., Pfeifer, A., & Fässler, R.  (2006). Cyld inhibits tumor cell proliferation by blocking Bcl-3-dependent NF-kB signalling. Cell 2006; 125(4): 665-77.
  •  Pfeifer, A., Eigenbrod, S., Al-Khadra, S., Hofmann, A., Mitteregger, G., Moser, M., Bertsch, U., & Kretzschmar, H. (2006). Lentivector-mediated RNAi efficiently suppresses PrP and prolongs survival of scrapie-infected mice. Journal of Clinical Investigation. 2006; 116(12):3204-10
  • Pfeifer, A., Ikawa, M., Dayn, Y., & Verma, I.M. (2002). Transgenesis by lentiviral vectors: Lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos. Proc. Natl. Acad. Sci. USA. 2002; 99:2140-2145.

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